Review



pcold gst  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    TaKaRa pcold gst
    Pcold Gst, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst/product/TaKaRa
    Average 95 stars, based on 40 article reviews
    pcold gst - by Bioz Stars, 2026-02
    95/100 stars

    Images



    Similar Products

    pcold gst  (TaKaRa)
    95
    TaKaRa pcold gst
    Pcold Gst, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    pcold gst - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    pcold gst plasmid  (TaKaRa)
    95
    TaKaRa pcold gst plasmid
    PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into <t>pCold</t> TF plasmid and Catch was cloned into pCold <t>GST</t> plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.
    Pcold Gst Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst plasmid/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    pcold gst plasmid - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    pcold gst dna  (TaKaRa)
    95
    TaKaRa pcold gst dna
    PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into <t>pCold</t> TF plasmid and Catch was cloned into pCold <t>GST</t> plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.
    Pcold Gst Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst dna/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    pcold gst dna - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    pcold gst 3372 plasmid  (TaKaRa)
    95
    TaKaRa pcold gst 3372 plasmid
    PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into <t>pCold</t> TF plasmid and Catch was cloned into pCold <t>GST</t> plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.
    Pcold Gst 3372 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst 3372 plasmid/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    pcold gst 3372 plasmid - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier
    pcold gst vector  (TaKaRa)
    95
    TaKaRa pcold gst vector
    PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into <t>pCold</t> TF plasmid and Catch was cloned into pCold <t>GST</t> plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.
    Pcold Gst Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcold gst vector/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    pcold gst vector - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into pCold TF plasmid and Catch was cloned into pCold GST plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.

    Journal: Journal of Virology

    Article Title: Midnolin inhibits coronavirus proliferation by degrading viral proteins

    doi: 10.1128/jvi.00366-25

    Figure Lengend Snippet: PEDV S1/S2/M/E proteins are degraded by autophagy via interaction with MARCH8 and Tollip. ( A and B ) HEK-293T cells were subjected to transfection with plasmids encoding Flag-Catch and MYC-MARCH8 or HA-Tollip for 24 h and subsequently explored with Co-IP assay. β-Actin was used as the sample loading control. ( C and D ) The MARCH8 and Tollip were cloned into pCold TF plasmid and Catch was cloned into pCold GST plasmid, respectively. Recombinant proteins were indicated in bacterial strain BL21 (DE3) and purified for the GST pulldown analysis. ( E–H ) HEK-293T cells were transfected with plasmids encoding Flag-Tollip and HA-S1/S2/M/E for 24 h and subsequently explored with Co-IP assay. β-Actin served as the sample loading control. ( I ) HeLa cells were exposed to transfection with plasmids encoding Flag-Tollip and HA-S1/S2/M/E, Flag-Catch and MYC-MARCH8, or Flag-Catch and HA-Tollip. Additionally, the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Meanwhile, the colocalization of Tollip and S1/S2/M/E, Catch and MARCH8, or Catch and Tollip was observed with the use of confocal immunofluorescence microscopy; scale bars: 100 µm.

    Article Snippet: The full-length MIDN , PEDV S1/S2/M/E , MARCH8, and Tollip were cloned into pCold GST plasmid (Clontech Laboratories, Inc., 3372) or pCold TF plasmid (Clontech Laboratories, Inc., 3365), respectively.

    Techniques: Transfection, Co-Immunoprecipitation Assay, Control, Clone Assay, Plasmid Preparation, Recombinant, Purification, Staining, Immunofluorescence, Microscopy